Congratulations go to Mike for being awarded a Lerner-Gray Grant for Marine Research from the American Museum of Natural History. Mike will use the grant to explore the use of Oxford Nanopore sequencing to conduct coastal gene expression surveys for various abundant SAR11 bacteria, including LD12. An excellent accomplishment!
Mike, Celeste, and Emily will all be presenting at the 2017 ASM Microbe conference in New Orleans this coming weekend. Here is the info to find them:
Poster Session: AES11 – Freshwater and Marine Microbiology I
Time: 12:15 PM – 2:15 PM
Cultivation and Ecology of Novel SAR11 Taxa from Coastal Louisiana Waters (#700), V. Celeste Lanclos
Metabolic and Physiological Flexibility in a Coastal Isolate from the OM252 Clade of Gammaproteobacteria (#712), Emily Nall
Symposium: 425 – Culturing the Unculturable in a Sequencing Age (Room 352)
Time: 2:30 PM – 5:00 PM
Fresh and Salty: Cultivating Bacteria from the Coast of Louisiana, Michael W. Henson
For the past four months a crew of four rowers and four shore crew members with OAR Northwest, a not-for-profit adventure education organization, have been on a journey of a lifetime on the Mississippi River. After over 100 days of rowing, the crew has traveled from the headwaters of the River in Minnesota to the Gulf of Mexico. They arrived in Baton Rouge on November 16, 2016 and spent a few days visiting LSU and talking to students about their journey.
Just as the state of Louisiana has a special connection with the “Mighty” Mississippi River, the OAR Northwest rowing crew has a special connection with LSU. This is the second OAR Northwest Mississippi River adventure during which rowers have collected water samples for Dr. Cameron Thrash, an assistant professor in the LSU Biological Sciences department. Cameron’s research focuses on relationships between microorganisms and biogeochemical cycles, particularly in marine systems. Thanks to a relationship with the OAR Northwest team that started when founder Jordan Hanssen met Cameron’s family in Washington, and which has developed into an ongoing citizen science project, the Thrash lab is now building a complete microbial “map” of the Mississippi river…
It is with great excitement that I get to post that our manuscript on cultivating members of the microbial majority using an artificial seawater medium is finally out! This manuscript represents the hard work of not just myself, but Dr. Thrash, our undergraduates (past and present), and Austen Webber. Over the last two years, I have traveled to sites along the Gulf of Mexico collecting water for cultivation experiments (> 2000 miles traveled, > 4500 well inoculated). From the sites along the coasts of Louisiana, we have cultivated organisms from the Gulf of Mexico representing many important marine clades: SAR11, SAR116, OM43, OM252, Roseobacter, and many more. While isolating these organisms is important, it is also important to isolate organisms that represent abundant taxa within your source water. We compared OTUs from community sequencing of the source water to our isolate sequences to demonstrate that our method frequently captured some of the most abundant organisms in the system.
This work also represents the first instance where many of these clades were isolated from the Gulf of Mexico, and importantly, on an artificial seawater medium. While high throughput, dilution-to-extinction culturing using natural seawater has been highly successful, we hope that this new approach using artificial seawater media will help more researchers cultivate important microorganisms without the hassle of collecting large volumes of natural seawater and needing a boat.
If you have any questions, please feel free to contact us! We are more than willing to answer any questions you may have. You can check out our list of organisms isolated so far HERE!
Henson, Michael W., David M. Pitre, Jessica Lee Weckhorst, V. Celeste Lanclos, Austen T. Webber, and J. Cameron Thrash. (2016). Artificial seawater media facilitates cultivating members of the microbial majority from the Gulf of Mexico. mSphere 1(2). doi: 10.1128/mSphere.00028-16. (Undergraduate authors) Supplementary Information.
Some associated press became available on May 1st. Check out Becoming Acculturated, by Jeffrey M. Perkel.