Thrash Lab

Department of Biological Sciences, Louisiana State University


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Cultivating members of the microbial majority

It is with great excitement that I get to post that our manuscript on cultivating members of the microbial majority using an artificial seawater medium is finally out! This manuscript represents the hard work of not just myself, but Dr. Thrash, our undergraduates (past and present), and Austen Webber. Over the last two years, I have traveled to sites along the Gulf of Mexico collecting water for cultivation experiments (> 2000 miles traveled, > 4500 well inoculated). From the sites along the coasts of Louisiana, we have cultivated organisms from the Gulf of Mexico representing many important marine clades: SAR11, SAR116, OM43, OM252, Roseobacter, and many more. While isolating these organisms is important, it is also important to isolate organisms that represent abundant taxa within your source water. We compared OTUs from community sequencing of the source water  to our  isolate sequences to demonstrate that our method frequently captured some of the most abundant organisms in the system.

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This work also represents the first instance where many of these clades were isolated from the Gulf of Mexico, and importantly, on an artificial seawater medium. While high throughput, dilution-to-extinction culturing using natural seawater has been highly successful, we hope that this new approach using artificial seawater media will help more researchers cultivate important microorganisms without the hassle of collecting large volumes of natural seawater and needing a boat.

If you have any questions, please feel free to contact us! We are more than willing to answer any questions you may have. You can check out our list of organisms isolated so far HERE!

Henson, Michael W., David M. Pitre, Jessica Lee Weckhorst, V. Celeste Lanclos, Austen T. Webber, and J. Cameron Thrash. (2016). Artificial seawater media facilitates cultivating members of the microbial majority from the Gulf of Mexico. mSphere 1(2). doi: 10.1128/mSphere.00028-16. (Undergraduate authors) Supplementary Information.

Some associated press became available on May 1st. Check out Becoming Acculturated, by Jeffrey M. Perkel.


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Antarctica research published

On March 23rd, research on the microbial variation across a 5500 km transect of Antarctic surface sediment that Dr. Thrash and I had worked on with Dr. Deric Learman from Central Michigan University was finally published in Frontiers in Microbiology under the special topic: Microbiology of the rapidly changing polar environments. Since then, the article has had >1200 views from around the globe and was one of the top ten articles in Frontiers in Microbiology for the month of March. The research began when I was a Masters student in Dr. Learman’s lab. When I came here to LSU, Dr. Thrash was added to the project. This research would of never happened without the help of Dr. Andrew Mahon (CMU), Dr. Scott Santos (Auburn), Dr. Kenneth Halanych (Auburn), and Dr. Pamala Brannock (Auburn). Each one helped collect our sediment samples while they were out to sea doing their own research. I’d also like to thank Dr. Ben Temperton (University of Exeter) who helped with our analyzes.  We are excited to finally have it published!

Here is a quick blurb on it:

Western Antarctica, one of the fastest warming locations on Earth, is a unique environment that harbors under explored levels of biodiversity. Our work focuses on the seemingly “invisible” inhabitants of the ocean floor that boarder the western and peninsula portion of the Antarctic continent.   While microorganisms are the smallest forms of life on Earth, they are abundant (typically more than 10 million cells per gram of sediment) and influence the cycling of important nutrient such as carbon and nitrogen. They also represent the foundation of the food chain that supports larger and more complex forms of life. To study this environment, ocean sediment samples from the continental shelf of western Antarctica were collected over a 5,500 km transect from the Ross Sea to the Weddell Sea. By using 16S and 18S rRNA amplicon sequencing, this work has shown these sediments to be incredibly diverse and were distinguished by their correlations to organic matter and stable isotope fractions (TN, δ13C, etc.). Our work further demonstrates the versatility of marine microbial life and its ability to persist at near zero temperatures as well as greatly increases the available information for this region.

Learman, Deric R., Michael W. Henson, J. Cameron Thrash, Ben Temperton, Pamela M. Brannock, Scott R. Santos, Andrew R. Mahon, and Kenneth M. Halanych. (2016)
 Biogeochemical and microbial variation across 5500 km of Antarctic surface sediment implicates organic matter as a driver of benthic community structureFrontiers in Microbiology7:284. doi: 10.3389/fmicb.2016.00284


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Emily and Celeste present at LSU Discover Day

IMG_7074Emily presented her LA Sea Grant-funded work to characterize one of our coastal isolates from the OM252 clade, LSUCC0096. She showed this organism has remarkable salinity tolerance, and can grow under chemolithoautotrophic conditions, a feature that was predicted from genome analysis.

 

 

 

 

IMG_7071Celeste presented results of her experiment to enrich for microorganisms that could utilize fulvic acids as their sole carbon source. She compared her work to that of former lab member Jessica Weckhorst who performed a similar experiment with humic acids. These are both extremely important fractions of the marine DOC pool.


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Sampling the Mississippi River Delta

Mike and I went on a sampling trip Tuesday (1/12/16) to the Mississippi Delta (“The Birdfoot”) near Buras, LA. This was my first sampling trip ever with the Thrash lab, and I must say that it was a great experience! We headed out loaded up with our equipment and coffee, and ended up with a fantastic view of  the sunrise at the edge of Lake Pontchartrain. Sadly, the picture does not come close to the beauty of  our actual view.

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Sunrise at the edge of Lake Pontchartrain

At the site, Mike waded out into the water to collect the sample while I got to hang back and enjoy the scenery. It was a great day to be outdoors with cool air and the sun shining.

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Our view from the dock

 

Some pelicans that didn't seem to mind us

Some pelicans that didn’t seem to mind us

 

Mike taking YSI readings

Mike taking YSI readings

 

Mike filtering a water sample

Mike filtering a water sample

Overall, I really enjoyed my sampling adventure with Mike. This trip was a part of our 3 year study of the coastline to characterize the microbial population of the Louisiana coast in conjunction with adding microbes to our Louisiana State culture collection (LSUCC) from the Gulf of Mexico. I’m hopeful that we will isolate some novel organisms from this and future trips. Not all undergraduates are lucky enough to be a part of a lab that allows us to be out in the field for data collection, so I’m grateful to Dr. Thrash and Mike for the opportunity!

 


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Ending the first Diel sampling set


Enjoy some time pictures from time points 0200 and 0600 as we completed our 24 hour survey at the C transect site. The video I have uploaded is of the Platform and its fog horn that sounds every 20 seconds or so. Its a real joy to work and sleep to.

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The sunrise at 0600 this morning. To be cheesy #nofilter

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Sunrise on the Northern Gulf of Mexico after our last CTD cast

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The rain has come back on our way to the 2nd 24 hour survey site