Storify on finishing bacterial genomes

Usually when you sequence bacterial genomes, the process of completely closing them (so-termed because most bacterial genomes are circular) whereby a single scaffold overlaps itself at the ends, requires a lot of effort. We’ve had very good luck with our Illumina sequencing and SPAdes assembly process, negating the need for things like primer walking, and we’re […]

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Storify about freeze-thaw selection on cultures

There was a twitter conversation today about the relative changes that occur during domestication of bacteria. One element of the thread was the degree to which freezing (and thawing) imposes selection on cultures. I collected all the tweets in this regard. Lots of great info from Richard Lenski. [View the story “Freeze-thaw selection on cultures?” […]

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