Usually when you sequence bacterial genomes, the process of completely closing them (so-termed because most bacterial genomes are circular) whereby a single scaffold overlaps itself at the ends, requires a lot of effort. We’ve had very good luck with our Illumina sequencing and SPAdes assembly process, negating the need for things like primer walking, and we’re […]
Log vs. linear growth
Cultivation
Growth curves from 61 isolates (in duplicate) after their first transfer from Mike’s last HPC experiment.
Dilution to extinction….
-jct
Thrash Lab Sampling at Calcasieu Lake Jetties
About a month ago, David Pitre (Thrash Lab undergraduate) and I went on our first excursion to collect Gulf of Mexico water samples as part of the LSU Culture Collection and surveying of the microbial communities of Southern Louisiana coastline grant. The Gulf of Mexico offers a vast realm of sampling sites for a microbiologist. […]
Storify about freeze-thaw selection on cultures
There was a twitter conversation today about the relative changes that occur during domestication of bacteria. One element of the thread was the degree to which freezing (and thawing) imposes selection on cultures. I collected all the tweets in this regard. Lots of great info from Richard Lenski. [View the story “Freeze-thaw selection on cultures?” […]
Storify on nucleic acid extraction methods
Let’s face it. There are a lot of different ways to skin a microbe, and get the DNA and RNA out for whatever purposes you might deem necessary or useful. If you ask one or two people you’ll get one or two answers. What if you ask more than a thousand? Here is some insightful crowd-sourced […]
