Sampling at the Calcasieu Jetties

On Monday, January 15th, I got to join Celeste on a sampling trip to Calcasieu Jetties in Cameron, Louisiana. This was my first time going on a sampling trip since joining the Thrash Lab. While it was quite a cold morning, it was still a fun time. There were some beautiful views along the way of Louisiana’s marshes.

View of the marshes

Once we got to the sampling site, Celeste had the joy of wading out into the cold water and collecting.  At least it was a beautiful morning with the sun shining down and bright blue skies.

The sampling site
Celeste striking a victory pose after collecting the water sample
Celeste filtering the sample

The purpose of this trip was to obtain a water sample for the mCURE sections of introductory biology lab. Using this sample, the students will learn high throughput cultivation protocols used in the Thrash Lab. I’m happy to have been able to help with the fieldwork and hope that the students enjoy learning about what our lab does.

Thrash Lab presentations at Ocean Sciences

We’re going to Ocean Sciences in Portland next week. Both Celeste and Mike have posters on Thursday, February 15th from 1600-1800:

MM44A-1512: The Ecology, Physiology, and Genomic Analysis of Novel SAR11 Isolates

MM44A-1513: Cultivation, genomics, and characterization of the first isolate from the freshwater SAR11 clade LD12

Session MM44A: Functional, Ecological, and Evolutionary Implications of Microdiversity and Intraspecific Variability in Aquatic Microorganisms I.


Diving the Dead Zone

Back in August, I accompanied Nancy Rabalais and LUMCON dive safety officer Ben Acker on a dive trip to station C6C. That location is an oil platform south of Terrebonne Bay with equipment for monitoring water conditions such as temperature, salinity, and dissolved oxygen. I’ve posted before about our work there exchanging equipment and taking samples. I’m involved with the LUMCON dive team through my continued collaboration with Nancy in researching seasonal hypoxia (a.k.a. the Dead Zone) in the region. For example, see our most recent paper on dead zone microbiology. The purpose of this particular trip was to show CBS News the heart of the Dead Zone. Nancy’s recent NOAA-sponsored hypoxia cruise (see Celeste’s trip report) revealed that this year’s zone of hypoxia was the largest ever, and it has attracted a lot of attention as a result. Below is the full-length GoPro footage of the dive, in three parts. A big chunk of the second and third parts are in blackness, at the bottom of the dive, where we searched, in vain, for a lost piece of equipment. But there is some beautiful footage of the rest of the water column if you scroll through the individual videos. A portion of this was included in the CBS News profile. UPDATE 10/4/17: Times-Picayune reporter Sara Sneath found this post and put together a cool summary and link for us at



Thrash Lab Ph.D. position in aquatic microbiology

We’re looking for students interested in pursuing Ph.D. research on one of a variety of topics in marine and estuarine microbiology. Possible projects involve comparative genomics, integrative (meta)genomics and physiology, synthetic ecology, integrated transcriptomics and metabolomics, high-throughput cultivation, and host-phage interactions. Both basic and applied research avenues are available, and students will have the ability to customize their project based on their interests, including field work/ship time if desired. LSU hosts an advanced high-performance computing environment (, the Socolofsky Microscopy Center, and is an excellent institution for interdisciplinary research at the boundaries of microbiology, marine science, computer science, chemistry, and engineering.

The ideal student will have a positive, solution-minded attitude, be enthusiastic about learning, be kind and hard working, will enjoy pursing research in a collaborative environment, and meet the minimum admissions requirements for LSU. We are currently accepting students for Fall 2018. To be considered for a position, please first send a CV and a brief description of research interests to

The Dead Zone on CBS News

After the research cruise in which Celeste helped Nancy Rabalais and her team measure the largest Dead Zone yet, news agencies are taking notice. Yesterday I dove with Nancy and Ben Acker of LUMCON at a site in the heart of the Dead Zone, station C6C (featured in many previous posts). Nancy maintains multiple SONDEs on a leg of the oil platform to measure dissolved oxygen, temperature, salinity, and other important parameters. Our purpose yesterday was to search for a SONDE lost on a previous dive and introduce the CBS News team to the region of hypoxia. We also wound up providing footage for the CBS News crew to use in their segment that you can watch HERE. I shot the underwater footage. We’ll be posting the full video later. Here are some shots from the R/V Acadiana yesterday.

Station C6C under stormy skies.
Captain Carl and CBS News Producer Warren Serink look on as we approach C6C.
Through a condensation-laden window, you can just see Nancy interviewing with Jeff Glor on the left while cameraman Max Stacy gets additional footage.
On the drive out, rain on the western horizon.

Shelfwide Hypoxia Cruise 2017

Recently, I’ve been lucky enough to take part in Dr. Nancy Rabalais’ annual mapping of the hypoxic zone in the Gulf of Mexico. Dr. Rabalais has led this cruise for the past 31 years, and has graciously allowed me onboard the R/V Pelican for my first research cruise. This cruise set out July 31st and returned on August 1st.

The R/V Pelican in dock at LUMCON before departure.

My primary role was to collect surface water at each site that will be used by the Rabalais Lab for water nutrient and suspended sediment data. This consisted of throwing the infamous bucket overboard:

Water collection with the surface bucket.

While onboard, I also was able to collect some samples for the Thrash Lab. I sampled from stations nearshore on transects that extended outward from our Board of Regents coastal sites that we have been visiting for the past few years (check out Henson et al. 2016 for more info). I collected surface water via the CTD from sites of interest and used a peristaltic pump to size fraction the water through 2.7um and 0.2um filters. These filters will be used to study the community composition of sites offshore in relation to the three year data that we already have from our coastal sites.

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My filtering set-up in one of the labs onboard. The seas were mostly calm, so I was able to only lightly strap my materials.
LSU Research Assistant Tom (left) and LSU undergraduate Ethan (right) casting the CTD. Notice the lovely view at midnight.

The first day of the cruise was a bumpy ride, and I was a little seasick despite the motion sickness medicine. Gladly, the waves soon settled, and the rest of the cruise was smooth sailing! I was on the night shift (7pm-7am), which meant that we got to see all of the cool fauna! The floodlight that followed the CTD into the water was a beacon for sealife, and we saw jellyfish, eels, squid, dolphins, crabs, and fish off all kinds. The fauna actually became an indicator of the dissolved oxygen in the bottom waters. Often when we found crabs and eels swimming on the surface, the bottom waters had very low oxygen levels or were virtually anoxic. While we waited for the CTD to come back onboard, we sometimes netted some Sargassum to see what kind of critters were living there.

Cute fish that we pulled from the Sargassum (we threw it back).

One of my favorite parts about the trip was the spectacular views of the sky. At night on the shelf, the only lights come from oil rigs. Sometimes we were quite close to these rigs…

Oil rig next to our C6C sampling site. It was really neat to see one this close.
…while other times we were pretty far from their light pollution. When we found ourselves distanced from the rigs, the stars lit up the sky, and the Milky Way was visible from the bow of the ship. We often laid there gazing upwards and talking about our lives and how the open sky humbled our problems. I didn’t have a camera capable of imaging the night sky, but I did get some shots of the sunrises and sunsets that we witnessed:
Sunset from the bow of the boat.
Probably the most vivid sunset of the trip.
Sunrise from the work deck.
Overall, this was both a great sampling trip and an amazing life experience. I bonded with the science and boat crew, and learned so much from Dr. Rabalais, Dr. Turner, and Wendy. I am thankful for this opportunity, and eager to be at sea again.
The highly fashionable safety gear required on the work deck.
Check out for participants, daily logs, images, site maps, and results of this cruise (spoiler: the hypoxic zone this year is record breaking!), and follow me @vclanclos on twitter!