The Thrash lab investigates the relationships among and between aquatic microorganisms and their environment. We combine cultivation independent methods (e.g., metagenomics, metatranscriptomics, and single-cell genomics) with high throughput culturing, physiology, and microbial genomics, and comparisons to chemical and physical measurements. We seek to understand why microorganisms occur where they do and what they are doing there. See our Publications page to get a sense of our recent work.
High throughput culturing
We conduct high throughput cultivation (HTC) experiments using tailored, defined media from a number of environments, including the coastal Gulf of Mexico, Lake Michigan, the Eastern Tropical North Pacific, and the San Pedro Ocean Time Series. We also collect water chemistry and microbial community data from the source water to evaluate cultivation efficacy and refine our defined media recipes. To date, we have isolated over 400 microorganisms, many of which represent some of the most abundant taxa in the sampled environments. Prominent examples include the first isolation of a freshwater SAR11 (a.k.a. LD12) representative, members of SAR11 subclade IIIa, OM43, OM182, SAR92, SAR116, Limnohabitans, Polynucleobacter, Actinobacter acIV, and many others.
Isolates obtained via HTC that have prominent roles in their community, are phylogenetically novel, and/or have the potential to contribute to our understanding of ecosystem functions are investigated further. We conduct a battery of physiological experiments to determine optimum growth conditions, comparative genomics to facilitate metabolic reconstruction, and genome-informed tests of metabolic capability. Isolates from the SAR11, SAR116, and OM252 subclades are currently under investigation, and we have many others on deck!
Evolution of salinity tolerance in SAR11
During 2018-2019, in collaboration with Liz Kujawinski (WHOI), we will be quantifying the mechanistic differences between sister clades of SAR11 that have evolved to inhabit environments of with very different ionic strength regimes. Stay tuned for more!
Microbial ecology of the northern Gulf of Mexico “dead zone”
We collaborate with Nancy Rabalais (LSU/LUMCON), Olivia Mason (Florida State University), and Brett Baker (University of Texas) in deploying a wide range of cultivation-independent techniques to learn about the microbial communities and genetics associated with the generation and maintenance of seasonal bottom water hypoxia on the Northern Gulf of Mexico shelf region (see lab blog entries for 2013 and 2014 cruise information).
Microbial ecology of the Mississippi River
We have been coordinating with OAR Northwest in a citizen science and education program to explore the microbial assemblages inhabiting the Mississippi River and begin quantifying their relationship to eutrophication. To date, we have completed two full-length Mississippi River transects using rowboats, and are in the process of publishing our results. You can read more about the science HERE and watch a promotional video from the 2014 season: