Congratulations go to Mike for being awarded a Lerner-Gray Grant for Marine Research from the American Museum of Natural History. Mike will use the grant to explore the use of Oxford Nanopore sequencing to conduct coastal gene expression surveys for various abundant SAR11 bacteria, including LD12. An excellent accomplishment!
Storify on finishing bacterial genomes
Usually when you sequence bacterial genomes, the process of completely closing them (so-termed because most bacterial genomes are circular) whereby a single scaffold overlaps itself at the ends, requires a lot of effort. We’ve had very good luck with our Illumina sequencing and SPAdes assembly process, negating the need for things like primer walking, and we’re […]
Ending the first Diel sampling set
Enjoy some time pictures from time points 0200 and 0600 as we completed our 24 hour survey at the C transect site. The video I have uploaded is of the Platform and its fog horn that sounds every 20 seconds or so. Its a real joy to work and sleep to. And so end time […]
Dilution to extinction….
-jct
Thrash Lab Sampling at Calcasieu Lake Jetties
About a month ago, David Pitre (Thrash Lab undergraduate) and I went on our first excursion to collect Gulf of Mexico water samples as part of the LSU Culture Collection and surveying of the microbial communities of Southern Louisiana coastline grant. The Gulf of Mexico offers a vast realm of sampling sites for a microbiologist. […]
Storify on nucleic acid extraction methods
Let’s face it. There are a lot of different ways to skin a microbe, and get the DNA and RNA out for whatever purposes you might deem necessary or useful. If you ask one or two people you’ll get one or two answers. What if you ask more than a thousand? Here is some insightful crowd-sourced […]
Storify on storing nucleic acids in unfriendly environments
Sometimes you want to collect samples in places that are hard to get to, or get out of. Sometimes those places are hot. Sometimes the people collecting your samples are not you, and they don’t have a lot of training. Sometimes there isn’t a freezer nearby. Or even electricity. Does this mean you can’t get […]
